Postprandial lipid metabolism durably enhances T cell immunity

Ethics and study approval All experiments detailed in this manuscript were approved by the University of Pittsburgh Institutional Animal Care and Use Committee (20077737 and 23073380). The use of retroviral vectors was approved by the Institutional Biosafety Committee. Research involving human participants was approved by the institutional review board (21090049). Animals C57BL/6J inbred mice were purchased from Jackson Laboratories. Experimental mice were maintained under specific-pathogen-free conditions in accordance with the University of Pittsburgh Institutional Animal Care and Use Committee, certified by the Association for Assessment and Accreditation of Laboratory Animal Care. Procedures were performed under their guidelines. Male and female, 6- to 8-week-old mice were used in all the experiments. C57BL/6J mice were bred in-house, at a Charles Rivers facility (starting October 2020), or obtained from the Jackson Laboratory. NOD.Cg-Prkdc scid ll2rg tm1Wjl /SzJ/Arc (NSG), Rag2 −/− and Ldlr −/− mice were purchased from the Jackson Laboratory. Cell lines and cell culture B16, B16-OVA, A549 and NALM6 cell lines were cultured in complete RPMI media (RPMI (Gibco, 11875-093), 10% fetal bovine serum (FBS), 2 mM l -glutamine, 100 U ml −1 penicillin–streptomycin, 1× non-essential amino acids, 1 mM sodium pyruvate, 5 mM HEPES, β-mercaptoethanol). …