Prime assembly with linear DNA donors enables large genomic insertions
Nature News ·

Suzuki, K. et al. In vivo genome editing via CRISPR/Cas9 mediated homology-independent targeted integration. Nature 540 , 144–149 (2016). …
Suzuki, K. et al. In vivo genome editing via CRISPR/Cas9 mediated homology-independent targeted integration. Nature 540 , 144–149 (2016). Article ADS CAS PubMed PubMed Central Google Scholar Yamamoto, Y. & Gerbi, S. A. Making ends meet: targeted integration of DNA fragments by genome editing. Chromosoma 127 , 405–420 (2018). Article CAS PubMed PubMed Central Google Scholar Anzalone, A. V. et al. Programmable deletion, replacement, integration and inversion of large DNA sequences with twin prime editing. Nat. Biotechnol. https://doi.org/10.1038/s41587-021-01133-w (2021). Lin, Q. et al. High-efficiency prime editing with optimized, paired pegRNAs in plants. Nat. Biotechnol. 39 , 923–927 (2021). Article CAS PubMed Google Scholar Jiang, T., Zhang, X.-O., Weng, Z. & Xue, W. Deletion and replacement of long genomic sequences using prime editing. Nat. Biotechnol. 40 , 227–234 (2022). Article CAS PubMed Google Scholar Wang, J. et al. Efficient targeted insertion of large DNA fragments without DNA donors. Nat. Methods 19 , 331–340 (2022). Article CAS PubMed Google Scholar Landrum, M. J. et al. ClinVar: public archive of relationships among sequence variation and human phenotype. Nucleic Acids Res. 42 , D980–D985 (2014). Article CAS PubMed Google Scholar Jasin, M. & Haber, J. E. The democratization of gene editing: insights from site-specific cleavage and double-strand break repair. DNA Rep. 44 , 6–16 (2016). …
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