Translation-dependent degradation of <i>cas12</i> mRNA triggered by an anti-CRISPR

Murtazalieva, K., Mu, A., Petrovskaya, A. & Finn, R. D. The growing repertoire of phage anti-defence systems. Trends Microbiol. 32 , 1212–1228 (2024). Article CAS PubMed Google Scholar Mayo-Muñoz, D., Pinilla-Redondo, R., Camara-Wilpert, S., Birkholz, N. & Fineran, P. C. Inhibitors of bacterial immune systems: discovery, mechanisms and applications. Nat. Rev. Genet. 25 , 237–254 (2024). Article PubMed Google Scholar Davidson, A. R. et al. Anti-CRISPRs: protein inhibitors of CRISPR-Cas systems. Annu. Rev. Biochem. 89 , 309–332 (2020). Article CAS PubMed PubMed Central Google Scholar Marino, N. D. Phage against the machine: discovery and mechanism of type V anti-CRISPRs. J. Mol. Biol. 435 , 168054 (2023). Article CAS PubMed Google Scholar Knott, G. J. et al. Broad-spectrum enzymatic inhibition of CRISPR-Cas12a. Nat. Struct. Mol. Biol. 26 , 315–321 (2019). Article CAS PubMed PubMed Central Google Scholar Zhang, H. et al. Structural basis for the inhibition of CRISPR-Cas12a by anti-CRISPR proteins. Cell Host Microbe 25 , 815–826.e4 (2019). Article CAS PubMed Google Scholar Marino, N. D. et al. Discovery of widespread type I and type V CRISPR-Cas inhibitors. Science 362 , 240–242 (2018). Article ADS CAS PubMed PubMed Central Google Scholar Varadi, M. et al. AlphaFold Protein Structure Database: massively expanding the structural coverage of protein-sequence space with high-accuracy models. Nucleic Acids Res. 50 , D439–D444 (2022). …