Telomere-to-Telomere Assembly Using HERRO-Corrected Simplex Nanopore Reads

Telomere-to-telomere (T2T) phased assemblies are emerging as a benchmark for reference-quality genomes 1,17 , though they remain technically and financially demanding, particularly at scale. Generating such assemblies for diploid and polyploid genomes typically involves combining high-accuracy long reads, such as PacBio HiFi 16 or the now-deprecated ONT Duplex 2 reads, with ultra-long ONT Simplex reads. …