The mutational landscape of STING-induced immunity

Cell culture HeLa (CCL-2) cells were obtained from Sigma-Aldrich. HEK293T cells were a gift from D. Trono, originally purchased from the American Type Culture Collection. BJ-5ta cells were obtained from the American Type Culture Collection. THP-1-Dual STING 1 knockout cells were purchased from Invivogen (thpd-kostg). HeLa and HEK293T cells were cultured in Dulbecco’s Modified Eagle medium (DMEM) (Thermo Fisher Scientific, 41965039) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS) (Thermo Fisher Scientific, Gibco SKU, 10270106), 1% (v/v) penicillin (100 IU ml −1 )–streptomycin (100 μg ml −1 ) (BioConcept, 4-01F00-H), 2 mM l -glutamine (Thermo Fisher Scientific, 25030024). BJ-5ta cells were cultured in a mixed medium consisting of 80% of DMEM and 20% of Medium 199 (Thermo Fisher Scientific, 41150087) supplemented with 10% FBS, 2 mM l -glutamine and 1% (v/v) penicillin–streptomycin. THP-1 cells were cultured in Roswell Park Memorial Institute 1640 Medium (RPMI 1640) (Thermo Fisher Scientific, 21875034) containing 10% (v/v) heat-inactivated FBS, 25 mM HEPES (BioConcept, 5-31F00-H), 2 mM l -glutamine and 1% (v/v) penicillin–streptomycin. All cells were cultured at 37 °C and at atmospheric O 2 and 5% CO 2 . HeLa cGAS knockout cells were reported previously 38 , 61 . Cell lines were routinely tested and confirmed to be negative for mycoplasma contamination. …