Spatial distribution of the proteome in the human body and in cancers

Collection of tissue samples The autopsy samples involved in this study were collected from the body donation centre of Dalian Medical University and Shanghai Jiao Tong University. The post-mortem interval was within 10 h. The project was approved by the Ethics Committee of Dalian Medical University (institutional review board (IRB): Dalian Medical 2019-05) and Shanghai Jiao Tong University (IRB: 2022-A-01, 2021-03). Written informed consent was obtained from the legally authorized representatives for all donors. To ensure anatomical accuracy, we used (1) macrodissection by two experienced anatomists (included in the author list) following a standardized protocol; and (2) histopathological validation through H&E-stained formalin-fixed paraffin-embedded (FFPE) sections reviewed independently by two pathologists (included in the author list) for tissue types that might not be clearly partitioned at the anatomical level. Paired tumours and non-tumour samples from surgery were collected from Harbin Medical University Cancer Hospital (from December 2019 to December 2022) and Huashan Hospital (from January 2018 to December 2018). The project was approved by the Ethics Committee of Harbin Medical University Cancer Hospital (IRB: KY2019-08, KY2023-03 and KY2019-16) and Huashan Hospital (KY2021-064). Written informed consent for each patient was collected. The samples mentioned above were stored in FFPE format. …