Gene ancestries reveal diverse microbial associations during eukaryogenesis

Data retrieval, curation and proteome database reconstruction eTOLDB construction To guide dataset selection and LECA reconstruction, we used the phylogenetic backbone of the eTOL and the nine eukaryotic supergroups classification depicted in Supplementary Fig. 1a . This represented a consensus between recent eTOL reconstructions 54 , 55 , 56 , 57 , 58 and an operational definition of two stems and nine supergroups that largely reflected previous classifications 59 . …