Whole-genome duplication shaped cell-type evolution in the vertebrate brain

Vertebrate scRNA and snRNA atlas collection, filtering and preprocessing Cell atlases were retrieved from previous publications 2 , 3 , 4 , 5 . Low-quality cells in the human atlas were further filtered based on nCount (UMI) 0.1. We retained only TFs and defined an orthogroup as a conserved TF orthogroup for astrocytes versus ependymal cells if at least three (out of four) vertebrates contained these TFs. …