LASER couples damage sensing to ESCRT assembly for lysosome repair

Antibodies and chemicals Reagents were obtained from the following sources: antibodies against HA (3724S, clone C29F4; 1:1,000 dilution for western blot), LC3B (3868S, clone D11; 1:1,000 dilution for western blot), VINCULIN (13901S, clone E1E9V; 1:1,000 dilution for western blot), LAMTOR4 (13140S, clone D4P60; 1:300 dilution for immunofluorescence), HRS (15087S, clone D7T5N; 1:1,000 dilution for western blot), TOM20 (42406S, clone D8T4N; 1:1,000 dilution for western blot), ATG5 (2630S; 1:1,000 dilution for western blot), ATG13 (13468S, clone E1Y9V; 1:1,000 dilution for western blot) from Cell Signaling Technology; LAMP2 (sc-18822, clone H4B4; 1:300 dilution for immunofluorescence), CHMP1A (sc-271617, clone B-5; 1:300 dilution for immunofluorescence) from Santa Cruz Biotechnology; TFG (ab156866, clone EPR8766; 1:300 dilution for immunofluorescence, 1:1,000 dilution for western blot) and NPC1 (ab36983; 1:1,000 dilution for western blot) from Abcam; SEC31A (612350, clone 32; 1:300 dilution for immunofluorescence) from BD Biosciences; SEC31A (17913-1-AP;1:300 dilution for immunofluorescence), TSG101 (67381-Ig, clone 2B7G8; 1:1,000 dilution for western blot), ALIX (67715-Ig, clone 1H9D9; 1:1,000 dilution for western blot), PDCD6/ALG2 (12303-1-AP; 1:1,000 dilution for western blot) from Proteintech; TFG (NBP2-62212, clone TFG-03; 1:300 dilution for immunofluorescence) from Novus; LLOMe (16008) from Cayman; DMSO (D2650-100mL) from Sigma-Aldrich; Pierce Protease Inhibitor Tablets …