Plastoglobules compartmentalize nitrogen assimilation in maize

Plant materials The mutants nir2-1, nir2-2 , gln2 , gln3 , gln4 , gln5 and gln6 seeds were obtained from the mutant library ( http://maizeems.qlnu.edu.cn/ ) and generated in the WT (B73 background). The ZmNIR2 T1 OE and ZmNIR2 T2 OE overexpression lines were generated in the WT (B73 background). The mutants nir1 and gln1 were created by Center for Crop Functional Genomics and Molecular Breeding of China Agricultural University and generated in the WT (ND101 background). The inbred lines and teosinte accessions were propagated by our own research team. The ZmNIR2 T1 OE-12 and ZmNIR2 T1 OE-25 overexpression lines were generated in the WT (YH7 background). All the maize genetic variants were grown in the experimental fields in Wenjiang, Sichuan Province (30.7° N, 103.8° E), Sanya, Hainan Province (18.2° N, 109.3° E) and Harbin, Heilongjiang Province (44.0° N, 125.4° E). Genomic accession numbers for the genes studied are ZmNIR1 ( Zm00001eb255880 ), ZmNIR2 ( Zm00001eb193660 ), ZmGLN1 ( Zm00001eb432580 ), ZmGLN2 ( Zm00001eb054990 ), ZmGLN3 ( Zm00001eb399860 ), ZmGLN4 ( Zm00001eb253820 ), ZmGLN5 ( Zm00001eb190340 ) and Zm GLN6 ( Zm00001eb009090 ). Plant-growth conditions Maize seeds were disinfected with 75% ethanol for 3 min, followed by four rinses with deionized water. Disinfected seeds were then soaked in saturated calcium sulfate solution for 5 h and subsequently placed on moist absorbent paper in a dark, humid environment at 28 °C to induce germination. …