Eosinophils drive intestinal remodelling and innate defence in reproduction

Mice All animal experiment were performed in accordance with protocol approved by the Institutional Animal Care and Use Committee and Institutional Biosafety Committee at Princeton University. C57BL/6 (stock no. 000664), Lgr5 eGFP−IRES-creERT2 (stock no. 008875), BALB/cJ (stock no. 000651) and ΔdblGATA (stock no. 033551) mice were purchased from The Jackson Laboratory. Germ-free C57BL/6 mice were purchased from Taconic Biosciences. For timed pregnancy, females were intraperitoneally injected with 2 IU PMSG followed by 2 IU hCG 46–48 h later and then co-housed with male breeder mice. This hormone-induced timed pregnancy model was used for all experiments unless otherwise specified. For all experiments, C57BL/6 male breeder mice were used unless otherwise specified. Tissue collection and analyses were performed at embryonic day 12.5 for pregnant mice or 12 days postpartum for lactating mice unless otherwise specified. For long-term experiments, pups were weaned at 3 weeks and female mice were maintained for 12 weeks post-lactation. Age-matched mice that were administrated with hormones and co-housed with male mice but did not get pregnant were used in each experiment as nulliparous controls. Animals were randomly assigned to experimental groups whenever possible, and sample processing and outcome assessment were performed in a blinded manner where feasible. Bacteria and infections Wild-type Y. …