Large-scale discovery, analysis and design of protein energy landscapes

Library design The initial set of 15,715 domain sequences was organized into five batches and further divided into 18 libraries (mix 1–4, libraries 1 and 4; libraries 7–15; and mutants 2–4): (1) mix 1–4: de novo designed ααα, βαββ and ββαββ sequences 21 ; (2) libraries 1 and 4: de novo designed αββα proteins 11 ; (3) libraries 7–14: natural domains from the Pfam database, including LysM, PASTA, WW, SH3, pyrin and cold-shock; (4) library 15: PDB-derived monomeric proteins devoid of cysteine …