RNA-triggered cell killing with CRISPR–Cas12a2

Phylogenetic analysis The amino acid sequence of GeCas12a2 was aligned with other Cas12a2 nuclease sequences 15 , 59 using Clustal Omega 60 . The alignment was trimmed with ClipKIT 61 and used to reconstruct a phylogeny with IQ-TREE v2.0.3 (-m MFP -T 8 -B 1000) 62 , using a maximum-likelihood approach. The phylogeny included Cas12a orthologues and used Cas12c as an outgroup. Branch confidence was reported as ultrafast bootstrap values (ranging from 0 to 100). …