Expanding the human proteome with microproteins and peptideins

PeptideAtlas database construction and searching The human non-HLA PeptideAtlas 2023-06 build contains 295 ProteomeXchange datasets (PXDs) 50 split into 1,172 different experiments that comprise a total of 3.5 billion MS/MS spectra. Sequence database searching was performed using MSFragger 51 v.3.7 using search parameters appropriate for each dataset, depending on alkylation, labelling, fragmentation type, instrument, enrichment strategy and more. …